Material
▶TIANprep Mini Plasmid Kit (Gragen)
▶EasyPure® Plasmid MiniPrep Kit (Beijing TransGen Biotech)
▶Nanodrop (NanoPhotometers Spectrophotometers and Nanodrop, Implen)
▶cell cracker (JY92-2D, SCIENTIZ)
▶Ni2+-charged chromatography resin (GE Healthcare Company)
▶36MM dialysis bag (Biosharp)
▶light microscope (OLYMPUS BX61, TOKYO JAPAN)
▶Transmission Electron Microscope (TEM, H7650, Hitachi)
▶Dynamic Light Scattering(Zetasizer Nano ZS90, Malvern Panalytical)
▶circular dichroism (CD, PMS 450, Biologic)
▶ZR-ELP and PAP(purification assistant peptide) in pET-28a(+) (recombination plasmid, BGI)
▶(EK)10-TAT-LZE in pET-28a(+) (recombination plasmid, GENEWIZ)
▶AFIQ-BL21 strain E. coli (expression bacteria, GENEWIZ)